|
KMID : 0881720150300030289
|
|
Journal of Food Hygiene and Safety
2015 Volume.30 No. 3 p.289 ~ p.294
|
|
|
Development of Primer Sets for the Detection of Polygonum multiflorum, Cynanchum wilfordii and C. auriculatum
|
|
Kim Kyu-Heon
Kim Young-Sang
Kim Mi-Ra
Lee Ho-Yeon
Kang Tae-Sun
Lee Jin-Ha
Jang Young-Mi
Lee Kyu-Ha
Kim Jong-Hwan
Seong Rack-Seon
|
|
|
Abstract
|
|
|
|
The aim of this study was to develop rapid screening method for the identification of Chinese herbal medicine species with similar appearance, Polygonum multiflorum, Cynanchum wilfordii and C. auriculatum, by using genetic markers. As a genetic marker, psbA-trnH gene in chloroplast was selected due to differences in sequence among the three species. Species-specific primers were designed based on the sequences of the marker gene of P. multiflorum, C. wilfordii, and C. auriculatum, and the expected size of PCR products was 160, 147, and 119 bp, respectively. Under the developed conditions, cross-reaction was not detected among these three plant species. To confirm the efficiency of our species-specific primers, the optimized method was applied to a variety of processed products composed of mostly P. multiflorum and C. wilfordii, demonstrating that our method was a rapid and easy screening assay. Our findings suggest this screening method can be utilized to prevent the distribution of economically motivated adulteration food and to improve consumer's right.
|
|
|
KEYWORD
|
|
|
species-specific primer, PCR (polymerase chain reaction), adulterated food
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|